ABSTRACT
<p><b>OBJECTIVE</b>To observe T lymphocyte subsets and indicators of changes in viral load in sub-acute period in Chinese rhesus monkey model of AIDS SIVmac239. To explore Virology related index variation in sub-acute period of the Chinese rhesus monkey model of AIDS.</p><p><b>METHOD</b>To replicate Chinese rhesus monkey model of AIDS, healthy Chinese rhesus monkey was inoculated with SIVmac239 viral strain. To observe changes in T lymphocyte subsets indexes and viral load after infection with the simian immunodeficiency virus (SIV) in sub-acute period on an animal model. The clinical symptoms of the animal model was recorded simultaneously.</p><p><b>RESULT</b>During the 10 weeks after SIV acute infection, body weight and BMI index were relatively stable, the difference was not significant at all time points. Twelve monkeys were tested SIV positive by real-time PCR after three days of infection. On the 7th day after infection, 15 monkeys were tested SIV positive. Viral load increased rapidly, but reached a peak on the 10th-14th day after infection, then showed a level of volatility decline. T lymphocyte subsets showed significant changes, among them, CD3% and CD3 counts fluctuated upward trend and reached to the highest level in two weeks after infection; of CD4% and CD4 count changes were not synchronized, CD4% declined trend while the CD4 count was an increasing trend after the infection; of CD8% and CD8 counts fluctuate upward trend, and reached to a highest level in two weeks after infection ;the ratio of CD4/CD8 and the counts of CD4CD28 T cells decreased significantly in two weeks after infection; the former followed by a slow decline, the latter followed by a rapid rise. Three mouths after the infection 3 monkeys showed significant clinical symptoms. One of the rhesus monkeys had symptoms of diarrhea and two of them had reduced food intake.</p><p><b>CONCLUSION</b>This experiments established standardization of Chinese Rhesus monkeys used in the research of AIDS and provide a detailed contents in the changes of sub-acute phase.</p>
Subject(s)
Animals , Male , Acquired Immunodeficiency Syndrome , Acute Disease , Cell Count , Disease Models, Animal , Macaca mulatta , Simian Acquired Immunodeficiency Syndrome , Allergy and Immunology , Simian Immunodeficiency Virus , Physiology , T-Lymphocyte Subsets , Allergy and Immunology , Viral LoadABSTRACT
<p><b>OBJECTIVE</b>To observe the clinical effect on treatment of premature ovarian failure (POF) patients by Bushen Tiaojing Recipe (BTR) and hormone replacement therapy (HRT).</p><p><b>METHODS</b>Totally 72 POF patients were randomly assigned to three groups by random digit table, i.e., the Chinese medicine group, the Western medicine group, and the integrative medicine group, 24 in each group. Those in the Chinese medicine group took BTR. Those in the Western medicine group were treated by HRT. Those in the integrative medicine group were treated by BTR + HRT. All were treated for three courses and followed-up for 3 months after treatment. The clinical efficacy, integrals of clinical symptoms, and serum sex hormones levels [follicular stimulating hormone (FSH), estradiol (E2), luteinizing hormone (LH)] were compared among the three groups before treatment, at the end of treatment, and 3 months after withdrawal.</p><p><b>RESULTS</b>(1) The total effective rate was better in the integrative medicine group than in the Chinese medicine group and the Western medicine group (P < 0.05). (2) At the end of treatment, the integrals of clinical symptoms decreased in the 3 groups when compared with before treatment in the same group (P < 0.05, P < 0.01). The integrals of clinical symptoms were higher at 3 months after withdrawal than at the end of treatment in the Western medicine group (P < 0.05). But there was no statistical difference in changes of integrals between the Chinese medicine group and the integrative medicine group (P > 0.05). (3) By the end of treatment serum E2 increased (P < 0.01), FSH and LH decreased (P < 0.01) in the three groups, more significantly in the integrative medicine group and the Western medicine group (P < 0.05, P < 0.01). At 3 months after withdrawal serum E2 decreased, FSH and LH increased in the Western medicine group, showing statistical difference when compared with the other two groups (P < 0.05, P < 0.01). There was no statistical difference in changes of serum E2, FSH, or LH between the Chinese medicine group and the integrative medicine group (P > 0.05).</p><p><b>CONCLUSIONS</b>BTR combined HRT had significant effect on treatment of POF, could significantly improve patients' clinical symptoms, menstrual states, and serum sex hormones levels. It had lower recurrence rate. Patients suffered from less adverse reactions.</p>